Nuclease Methods and Protocols (Methods in Molecular Biology)
1st edition | English | February 15, 2001 | ISBN: 0896036790 | Pages: 525 | PDF | 5,1 mb
Nucleases, enzymes that restructure or degrade nucleic acid polymers, are vital to the control of every area of metabolism. They range from "housekeeping" enzymes with broad substrate ranges to extremely specific tools. Many types of nucleases are used in lab protocols, and their commercial and clinical uses are expanding.
The purpose of this book is to introduce the reader to some well characterized protein nucleases, and the methods used to determine their activity, structure, interaction with other molecules, and physiological role. Each chapter begins with a mini-review on a specific nuclease or a nuclease-related theme.